Respiration

Overview

What is respiration?

Remember in Photosynthesis, we saw that light energy was converted into chemical energy in the form of glucose.

But, cells cannot use glucose directly as a source of energy - they can only use ATP for their immediate energy needs.

Respiration is a process which breaks down the glucose, releasing energy to make this ATP

Why is respiration needed?

Since respiration produces ATP (immediate energy source for cells), it has many uses in cells for energy requiring process - see Hydrolysis & Synthesis ATP

Overview of Respiration

There are two main types of respiration

• Aerobic
  • Requires oxygen
  • Produces lots of ATP
  • Stages: Glycolysis, Link reaction, Krebs cycle & Oxidative Phosphorylation
• Anaerobic
  • Without oxygen
  • Produces minimal ATP
  • Stages: Glycolysis, Fermentation (Alcoholic for plants & yeast or Lactate for animals)

There are 4 main phases of aerobic respiration

• Glycolysis
• Link reaction
• Krebs cycle
• Oxidative phosphorylation

Glycolysis

Glycolysis occurs in the cytoplasm of the cell and doesn’t require oxygen (it’s a step in both aerobic and anaerobic). It produces pyruvate which can be transferred to the link reaction

Steps in Glycolysis

1. Phosphorylation to glucose phosphate
   • Glucose is phosphorylated using two ATP molecules to glucose phosphate
   • Note: Glucose phosphate is on the AQA specification, but the image here shows an additional step of hexose bisphosphate (with 2 phosphate groups on) - this is just for understanding of ‘where did the other phosphate from ATP go’?
2. Hexose bisphosphate is highly reactive and unstable so splits into 2 molecules of triose phosphate
3. Triose phosphate is oxidised and forms 2 molecules of pyruvate
   • Hydrogen is removed and transferred to NAD to form NADH (reduced NAD)
4. ATP is produced
   • 4 ATP is produced
   • Net gain of 2 ATP (as 2 were used up in phosphorylation step)

In aerobic respiration, the 2 molecules of pyruvate can be transported to the matrix of the mitochondria, for the link reaction

Active transport is required to transport pyruvate across the double membrane of the mitochondria.

Link Reaction

Steps in Link Reaction

• Pyruvate is oxidised to acetate, producing reduced NAD
• Acetate then combines with a coenzyme A, this forms acetyl coenzyme A (shortened to acetyl CoA)
• Products from the link reaction from 1 molecule of glucose (remember 1 molecule produces 2 pyruvates)
  • 2 acetyl CoA - this will feed into the Krebs cycle
  • 2 reduced NAD - this will go into oxidative phosphorylation
  • 2 carbon dioxide - produced as a waste product

Krebs Cycle

The Krebs cycle takes place in the mitochondrial matrix. It’s a series of oxidation-reduction reactions (redox).

Steps in Krebs Cycle

• Acetyl CoA (2C) reacts with a 4C molecule (Oxaloacetate), releasing coenzyme A and producing a six-carbon molecule (Citrate)

• The six-carbon molecule (Citrate) is converted to 5C molecule
  • Dehydrogenation = The hydrogen atoms are lost and are taken up by NAD to produce reduced NAD
  • Decarboxylation = carbon dioxide is removed (6C-> 5C)
• The 5C compound is converted to 4C (Oxaloacetate)
  • There are lots of intermediate compounds here (you don’t need to know these)
  • Decarboxylation = carbon dioxide is removed (5C->4C)
  • Dehydrogenation & oxidation of intermediate compounds
    • Hydrogen is removed and used to produce 1 x FAD reduced and 1 x NAD reduced
  • ATP is produced by substrate level phosphorylation
    • Phosphate group is directly transferred from one of the intermediates molecule to ADP to form ATP

Products from the Krebs Cycle

Important to note that for 1 molecule of glucose, this produces 2 x pyruvate, so 2 x Acetyl CoA, so the Krebs cycle goes round twice

• 4 x carbon dioxide (2 for each turn)
• 2 x ATP (1 for each turn)
• 6 x reduced NAD (3 for each turn)
• 2 x reduced FAD (1 for each turn)

What is substrate level phosphorylation?

The phosphate group is directly transferred from a substrate molecule to ADP to form ATP.

• It occurs in:
  • Glycolysis (oxidation step, where ATP is produced)
  • Krebs cycle (from 5C to 4C Oxaloacetate)

Oxidative Phosphorylation

Oxidative phosphorylation occurs in the inner mitochondria membrane and produces lots of ATP! (much more than the other steps) Many enzymes and proteins involved with this process, e.g ATP synthase are present here.

This process is very similar to Light Dependent Reaction

Hydrogen atoms provided by reduced NAD and FAD

• The reduced NAD and FAD transfer hydrogen atoms, which split into hydrogen ions (H+) and electrons to the electron transport chain. They are oxidised to become NAD and FAD.
  Where are the reduced NAD & FAD from?

  All the other steps of respiration, including Glycolysis, Link reaction, and Krebs cycle.

Electrons move down the electron transport chain

• Electrons lose energy at each carrier (move from a higher energy level to a lower energy level)
• This energy is used to pump the H+ ions out of the mitochondria matrix and into the intermembrane space.
• This establishes an electrochemical gradient, with a higher concentration of H+ ions in the intermembrane space than the matrix

Chemiosmosis

• As the hydrogen ions move down their concentration gradient, they pass through an enzyme called ATP synthase. This movement of hydrogen ions drives the synthesis of ATP (from ADP + Pi)

Oxygen is the final acceptor of electrons

• At the end of the electron transport chain, electrons, protons/H+ ions, combine with oxygen to form water.

Anaerobic Respiration

In anaerobic respiration, there isn’t oxygen to be the final acceptor in the electron transport chain, so we need an alternative way to breakdown glucose. This is where the fermentation pathway comes in.

The first step is Glycolysis which breaks glucose down to pyruvate This pyruvate can be used in either a:

• Yeast fermentation - where pyruvate is converted to ethanol
• Lactate fermentation - where pyruvate is converted to lactate

Other respiratory substrates

The food you eat doesn’t just contain glucose, it contains other nutrients such as fats and proteins.

These can be broken down into:

• Fats: Glycerol & fatty acids
• Proteins: Amino acids

These can be converted into molecules that can enter the Krebs cycle (usually Acetyl CoA)